Human Cytokine Array C1000

Antigen Information

Gene Symbols:

ADIPOQ | AGRP | ANG | ANGPT2 | AREG | AXL | BDNF | BMP4 | BMP6 | BTC   View more

Assay Format

Number of Targets Detected:

120

Species Detected:

• • • Human

Compatible Sample Types:

• • • Cell Culture Supernatants
    • Cell Lysates
    • Plasma
    • Serum
    • Tissue Lysates

Design Principle:

• • • Sandwich-based

Method of Detection:

Chemiluminescence

Quantitative/Semi-Quantitative:

• • • Semi-Quantitative

Solid Support:

Membrane

Product Specifications

Size:

2, 4, or 8 Sample Kit

Product Features

• • • Easy to use
    • No specialized equipment needed
    • Compatible with nearly any liquid sample
    • Proven technology (many publications)
    • Highly sensitive (pg/ml)
    • Sandwich ELISA specificity
    • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Species Detected

Human

Application Notes

Suggested Application

Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process

Kit Components

• • • Human Cytokine Antibody Array C1000 Membranes
    • Blocking Buffer
    • Wash Buffer 1
    • Wash Buffer 2
    • Biotinylated Detection Antibody Cocktail
    • Streptavidin-Conjugated HRP
    • Detection Buffer C
    • Detection Buffer D
    • Lysis Buffer
    • 8-Well Incubation Tray
    • Plastic Sheets
    • Array Templates
    • Manual

Other Materials Required

• • • Pipettors, pipet tips and other common lab consumables
    • Orbital shaker or oscillating rocker
    • Tissue Paper, blotting paper or chromatography paper
    • Adhesive tape or Saran Wrap
    • Distilled or de-ionized water
    • A chemiluminescent blot documentation system (such as UVP’s ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE’s ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Protocol Outline

• • 1. Block membranes
    2. Incubate with Sample
    3. Incubate with Biotinylated Detection Antibody Cocktail
    4. Incubate with HRP-Conjugated Streptavidin
    5. Incubate with Detection Buffers
    6. Image with chemiluminescent imaging system
    7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.

Not for diagnostic or therapeutic use.