Human HER2 / ErbB2 Protein (His Tag)
10004-H08H
Brand
Sino Biological
Description
Species : Human
Synonyms : CD340,ENV,ENVW,ERVWE1,HER-2,HER-2/neu,HER2,HERV-7q,HERV-W-ENV,HERV7Q,HERVW,HERVWENV,MLN 19,MLN19,NEU,NGL,TKR1
Host : HEK293 Cells
Purity :> 90 % as determined by SDS-PAGE
Activity : 1. Measured by its binding ability in a functional ELISA.
Immobilized human Erbb2 at 2.5 £gg/ml (100 £gl/well) can bind Herceptin with a linear range of 1.28-32 ng/ml.
2. Measured by its ability to block anti-ErbB2 mediated inhibition of BT474 human breast ductal carcinoma cell proliferation. The ED50 for this effect is 0.2-1 £gg/mL in the presence of 0.6 ?g/mL Anti-ErbB2/Her2 Monoclonal Antibody.
3. Using the Octet RED System, the affinity constant (Kd) of Her2 / ERBB2 Protein, Human, Recombinant (His Tag) (Cat: 10004-H08H) bound to Pertuzumab was 0.1 nM.
Application
Reactivity
