Activity : 1. Measured by its binding ability in a functional ELISA.
2.Immobilized human IL6 (Cat:GMP-10395-HNAE) at 10£gg/mL (100£gL/well) can bind human IL6R-His (Cat:10398-H08H) , the EC50 of human IL6R-His is 0.1-0.5 £gg/mL.
3. Measured in a cell proliferation assay using TF?1 human erythroleukemic cells. The ED50 for this effect is 0.2-0.8 ng/mL. The specific activity of recombinant human IL-6 is approximately 3.2 x 105 IU/£gg, which is calibrated against human IL-6 WHO International Standard (NIBSC code: 89/548).