Human Malignant Non-Hodgkin Lymphoma With Natural Killer Cells NK-92 MI
iCell-h331
Each vial contains >1 x 10^6 cells in 1ml volume
Brand
iCell Bioscience
Description
NK-92 cell is an IL-2-dependent NK cell line derived from peripheral blood mononuclear cells of a 50-year-old white male with aggressive non-Hodgkin’s lymphoma. NK-92 MI cells are IL-2-independent NK cell lines derived from NK-92 cells. The parent cell NK-92 is transformed by microsomal gene transformation using human IL-2CDNA carried by retrovirus MFG-HIL-2 vector. Transformation is stable, probably due to vector integration into genomic DNA. This cell line is cytotoxic to many malignant cells; Chromium release test shows that it can kill K562 cells and Daudi cells. NK-92 cells have the following characteristics: CD2, CD7, CD11a, CD28, CD45, CD54 surface marker shows positive; CD1, CD3, CD4, CD5, CD8, CD10, CD14, CD16, CD19, CD20, CD23, CD34 and HLA-DR surface marker shows negative. The parent IL-2-dependent cell line NK-92 and another IL-2-independent cell line NK-92CI derived from NK-92 cell line can be obtained from ATCC. Both NK-92MI cells and NK-92CI cells contain, express and synthesize HIL-2CDNA. NK-92MI cells produce a higher level of IL-2 than NK-92CI, while parental cells do not. The culture submitted to ATCC in September 1998 was contaminated with mycoplasma and its descendants were treated with BM cyclin for 21 days to eliminate mycoplasma. Six weeks after treatment, mycoplasma was tested by Hoechst staining, PCR and standard culture tests, and the results were negative.
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