Human MMP-9 Protein, His Tag

Source : 

Human MMP-9, His Tag (MM9-H5221) is expressed from human 293 cells (HEK293). It contains AA Ala 20 – Pro 469 (Accession # AAH06093).

Molecule : MMP-9

Synonyms : MMP9,CLG4B,GELB,MANDP2,Gelatinase B

Format : Powder

Category : Enzymes

Accession : AAH06093

Storage : -20℃

Shipping condition : Powder,RT

Molecular Weight : 50.8 kDa

Characteristics :

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 50.8 kDa. The protein migrates as 55-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

Buffer : 50 mM Tris, 150 mM NaCl, pH7.5

Description :

Matrix metallopeptidase 9 (MMP-9) is also known as 92 kDa type IV collagenase, 92 kDa gelatinase or gelatinase B (GELB), CLG4B, is secreted from neutrophils, macrophages, and a number of transformed cells, and is the most complex family member in terms of domain structure and regulation of its activity. . Structurally, MMP9 maybe be divided into five distinct domains: a prodomain which is cleaved upon activation, a gelatinbinding domain consisting of three contiguous fibronectin type II units, a catalytic domain containing the zinc binding site, a prolinerich linker region, and a carboxyl terminal hemopexinlike domain. This enzyme degrades various substrates including gelatin, collagen types IV and V, and elastin. MMP9 is involved in a variety of autoimmune diseases such as systemic lupus erythematosus, rheumatoid arthritis, and multiple sclerosis, and be regarded as a potential therapeutic target.

References :

(1) Hirose Y, et al., 2008, Am. J. Hum. Genet. 82 (5): 1122–9.
(2) Mira, E. et al., 2004, J. Cell. Sci. 117:1847-1857.