Human R-Spondin 1 / RSPO1 Protein (His Tag)

Species : Human

Synonyms : CRISTIN3,R-Spondin 1,RSPO

Host : HEK293 Cells

Purity :> 95 % as determined by SDS-PAGE

Activity : 1. Measured by its binding ability in a functional ELISA. Immobilized human RSPO1 at 20 £gg/ml (100 £gl/well) can bind human LIMPII with a linear range of 32-800 ng/ml.
2. Measured by its binding ability in a functional ELISA. Immobilized human RSPO1 at 20 £gg/ml (100 £gl/well) can bind mouse CD36 with a linear range of 6.4-800 ng/ml.
3. Measured by its ability to induce activation of ?catenin response in a Topflash Luciferase assay using HEK293T human embryonic kidney cells. The ED50 for this effect is typically 0.1-0.9 £gg/mL in the presence of 5 ng/mL recombinant mouse Wnt3a.