Human TFPI Protein (His Tag)

Species : Human

Synonyms : EPI,LACI,TFI,TFPI1

Host : HEK293 Cells

Purity :> 85 % as determined by SDS-PAGE

Activity : Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (Anaspec, Catalog#27114)
. The IC50 value is < 0.4 nM, as measured in 100£gL reaction mixture containing 1.25 ng trypsin (Sigma, Catalog#T4799), 10 £gM substrate, 50 mM Tris, 10 mM CaCl2, 0.15 M NaCl, 0.05% Brij-35, pH 7.5 .