IkBa Antibody

Brand

Description

pstrongAntibody Target :/strongIkBa/p
pbHost: /bMouse/p
pbAntibody Type: /bMonoclonal/p
pbIsotype: /bMouse IgG1/p
pbAntibody Form: /bAffinity Purified Antibody/p
pbImmunogen Sequence: /bRecombinant human IkBa/p
pbAccession#: /bP25963/p
pbGene ID: /b4792/p
pbAppearance: /bColorless liquid/p
pbConcentration: /b0.25 mg/ml/p
pbFormulation: /b100 micro;g (0.25 mg/ml) immunoaffinity purified mouse monoclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA, 0.02% thimerosal./p
pbPurification: /bAffinity purified/p
pbSpecies Reactivity: /bhu/p
pbApplication: /bWB/IP/IHC/p
pbApplication amp; Usage: /bWestern blotting (0.5-4 micro;g/ml) and immunoprecipitation (1-4 micro;g/ml). However, the optimal conditions should be determined individually. The antibody detects both phosphorylated and non-phosphorylated forms of IkBalpha;./p
pbStorage Temp.: /b-20 deg;C/p
pbShipping : /bgel pack/p
pbShelf Life: /b12 months/p
pbHandling: /bThe antibody solution should be gently mixed before use./p
pbBrief Background Descriptions: /bNF-kB is silenced in the cytoplasm by an inhibitory protein, IkB. IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/alpha;, IKK2/beta;, and IKK3/g. External stimuli such TNF-alpha; or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Six members of IkB family members have been identified. One of the first gene induced following NF-kB activation is IkBalpha;./p
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Application

Reactivity

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