ELISAInfluenza A H1N1 (A/Puerto Rico/8/1934) Hemagglutinin / HA ELISA Pair Set

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SEK11684

Decocription

分子 : HA

 

保存條件:

Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.

 

 

線性範圍 : 46.88-3000

 

包被抗體 : 0.5 mg/mL of mouse anti-H1N1 (A/Puerto Rico/8/1934) HA monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 μg/mL in PBS before coating. (Catalog: # 11684-MM05)

 

檢測抗體: 0.2 mg/mL mouse anti-H1N1 (A/Puerto Rico/8/1934) HA monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4). Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use. (Catalog: # 11684-MM03)

 

標準品 : Each vial contains 130 ng of recombinant H1N1 (A/Puerto Rico/8/1934) HA. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 3 ng/mL is recommended.

 

生物素交聯HRP:

 

靈敏度 : The minimum detectable dose of H1N1 (A/Puerto Rico/8/1934) HA was determined to be approximately 46.88 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.

 

特異性 :

 

檢測原理 : The H1N1 (A/Puerto Rico/8/1934) HA ELISA Pair Set is for the quantitative determination of H1N1 (A/Puerto Rico/8/1934) HA.
This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs.
The Sino Biological ELISA Pair Set is a solidphase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes amonoclonal antibody specific for H1N1 (A/Puerto Rico/8/1934) HA coated on a96-well plate. Standards and samples are added to the wells, and any H1N1 ( A/Puerto Rico/8/1934) HA present binds to the immobilized antibody. The wellsare washed and a horseradish peroxidase conjugated mouse anti-H1N1(A/Puerto Rico/8/1934) HA monoclonal antibody isthen added, producing an antibody-antigen-antibody “sandwich”. The wells areagain washed and TMB substrate solution is loaded, which produces color inproportion to the amount of H1N1 (A/Puerto Rico/8/1934) HA present in thesample. To end the enzyme reaction, the stop solution is added and absorbancesof the microwell are read at 450 nm.

 

檢測限: 46.88 pg/ml

 

血清樣本檢測範圍 :

 

Application

Reactivity

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