Influenza A H1N1 Nucleoprotein / NP Protein (His Tag)

Purity

> 97 % as determined by SDS-PAGE

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Activity

Testing in progress

Protein Construction

A DNA sequence encoding the Influenza A virus (A/Puerto Rico/8/34/Mount Sinai (H1N1)) nucleoprotein (AAM75159.1) (Met1-Gly490) was fused with a polyhistidine tag at the C-terminus.

GenBank#

Expressed Host

Baculovirus-Insect Cells

Species

H1N1

Predicted N Terminal

Met 1

Molecule Mass

The recombinant Influenza A virus (A/Puerto Rico/8/34/Mount Sinai (H1N1)) nucleoprotein comprises 501 amino acids and has a predicted molecular mass of 56.6 kDa. It migrates as an approximately 50 kDa band in SDS-PAGE under reducing conditions.

Formulation

Lyophilized from sterile 60mM Tris, 500mM NaCl, pH 7.4

1. Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

2. Please contact us for any concerns or special requirements.

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.

Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Stability & Storage

Samples are stable for up to twelve months from date of receipt at -70℃

Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.