Influenza A H7N9 (A/Anhui/1/2013) Hemagglutinin / HA Protein (His Tag)

Influenza A H7N9 (A/Anhui/1/2013) Hemagglutinin / HA Protein (His Tag): Product Information

Purity

> 95 % as determined by SDS-PAGE

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Activity

Measured by its ability to agglutinate guinea pig red blood cells.

HA titer is 0.5-2.5μg/mL for 1%GRBC

Protein Construction

A DNA sequence encoding the Influenza A virus (A/Anhui/1/2013(H7N9)) hemagglutinin (translated amino acid sequence of EPI439507) (Met1-Val524) was expressed with a C-terminal polyhistidine tag.

Accession#

EPI439507

Expressed Host

Baculovirus-Insect Cells

Species

H7N9

Predicted N Terminal

Asp 19

Molecule Mass

The recombinant hemagglutinin of Influenza A virus (A/Anhui/1/2013(H7N9)) comprises 517 amino acids and has a predicted molecular mass of 57.6 kDa. The apparent molecular mass of the protein is approximately 58 kDa in SDS-PAGE under reducing conditions.

Formulation

Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 7.4.

Please contact us for any concerns or special requirements.

Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

Please refer to the specific buffer information in the hard copy of CoA.