MICA Protein, Human, Recombinant (His Tag)

MICA Protein, Human, Recombinant (His Tag): Product Information

Purity

> 97 % as determined by SDS-PAGE

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Activity

Measured by its binding ability in a functional ELISA. Immobilized MICAh (Cat:12302-H08H1) at 10 μg/mL (100 μL/well) can bind S4-Fc3L3-NKG2D (Cat:10575-H01S), the EC50 of S4-Fc3L3-NKG2D (Cat:10575-H01S) is 200-450 ng/mL.

Protein Construction

A DNA sequence encoding the human MICA (AAH16929.1) extracellular domain (Met 1-Gln 308) was fused with a polyhistidine tag at the C-terminus.

Accession#

AAH16929.1

Expressed Host

HEK293 Cells

Species

Human

Predicted N Terminal

Glu 24

Molecule Mass

The recombinant human MICA consists of 296 amino acids and has a predicted molecular mass of 34 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh MICA is approximately 55-65 kDa due to glycosylation.