Myc-Ubiquitin

PDF

U-115

1 mg

Brand

Boston Biochem

Description

Myc-Ubiquitin

Catalog #: U-115

Myc-Ubiquitin
Product Source: 

human recombinant

This fully functional N-terminal myc-tagged ubiquitin protein allows for the convenient detection or affinity purification of ubiquitinated proteins in vitro. The tag sequence (EQKLISEEDL) is an epitope derived from the C-terminal region of the human proto-oncoprotein p62c-myc. This tag is specifically recognized by anti-myc antibodies or anti-myc-agarose. Ubiquitin is a 76 amino acid, highly conserved nuclear and cytoplasmic protein. It is found exclusively in eukaryotes, becomes covalently attached to substrate proteins by enzymes in the Ubiquitin-Proteasome Pathway (UPP) and has a major role in targeting cellular proteins for the ATP-dependent degradation by the 26S proteasome. Ubiquitination also affects proteasomeindependent events such as protein localization, activity and function.

Product Information
Quantity: 

1 mg

MW: 

9.3 kDa

Purity: 

> 95% by SDS-PAGE

Solubility: 

Aqueous solutions up to 10 mg/ml

Supplied: 

This is a lyophilized powder

 

Use & Storage
Use: 

Typical concentration to support in vitro conjugation is 500 μM to 1 mM depending on conditions

Storage: 

Lyophilized powder at 4 degrees C. Solubilized stock solution at -20 degrees C. Avoid multiple freeze/thaw cycles.

Application

Reactivity

Photos

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