NADP/NADPH Quantitation Colorimetric Kit

Alternate Name :

Tag Line :

No NAD or NADH Detection

Storage Conditions: -20°C

Shelf life: 12 months

Summary :

• Detection method- Absorbance (450 nm)
• Species reactivity- Mammalian
• Applications- Convenient tool for sensitive detection of the intracellular nucleotides: NADP, NADPH and their ratio

Detection Method:

Features & Benefits:

• Simple procedure; takes ~2 hours
• Fast and convenient
• Kit contains the necessary reagents for accurate measurement of NADP and NADPH and their ratio
• Enzymes in the system specifically recognize NADP/NADPH in an enzyme cycling reaction (It does not recognize NADᶧ/NADH)

Description:

Assays of nicotinamide nucleotides are of continual interest in the studies of energy transforming and redox state of cells or tissue. The NADP/NADPH Quantification Kit provides a convenient tool for sensitive detection of the intracellular nucleotides: NADP, NADPH and their ratio. The enzymes in the system specifically recognize NADP/NADPH in an enzyme cycling reaction (It does not recognize NADᶧ/NADH). There is no need to purify NADP/NADPH from sample mix. The enzyme cycling reaction significantly increases detection sensitivity. Results can be quantified using plate reader at OD450 nm.