NCP-CoV(2019-nCoV) Nucleoprotein / NP ORF mammalian expression plasmid (Codon Optimized)

Brand

Sino Biological

Description

NCP-CoV(2019-nCoV) Nucleoprotein / NP ORF mammalian expression plasmid (Codon Optimized): General Information
Gene
Species 2019-nCoV
RefSeq ORF Size 1260 bp
Sequence Description A number of silent mutations were introduced into the DNA sequence in order to increase its protein expression level in mammalian cell system. The translated amino acid sequence is identical with QHD43423.2 except for the point mutations: G355A.
Description Full length Clone DNA of 2019-nCoV Nucleoprotein / NP.
Plasmid
Promoter Enhanced CMV mammalian cell promoter
Vector pCMV3-untagged
Restriction Sites KpnI + XbaI(6.1kb+1.26kb)
Sequencing Primers T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Quality Control The plasmid is confirmed by full-length sequencing.
Screening
Antibiotic in E.coli Ampicillin
Antibiotic in Mammalian cell Hygromycin
Application Stable or Transient mammalian expression

Application

Reactivity

Photos