NK-92

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CLC027

Brand

gene-rl

Description

Cell Lines (GRL-CLC027)

Designations

NK-92

Cell type Human natural killer cell

Growth property

Suspension

Seeding density(cells/ml) 2X106

Survival rate

95%

Culture medium

75% Alpha minimum essential medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate with 0.2 mM inositol, 0.1 mM 2-mercaptoethanol, 0.02 mM folic acid, 100-200 U/ml recombinant IL-2 + 12.5% horse serum + 12.5% fetal bovine serum

Incubation condition

37°C，5% CO2

Freezed medium

90% culture medium + 10% DMSO

Medium renewal

every 2 to 3 days

Subculturing

 

Cultures can be maintained by addition or replacement of fresh medium.

Mycoplasma test

Negative for bacteria，fungi and mycoplasma

Advertence:

When you receive the cell line, please examine the flask and its cap. If it is not well-found, please tell us instantly. After examination, please wipe the flask and flask cap with 70% alcohol, and then loosen it. Afterward, put the flask into incubator immediately.

Application

Reactivity

Photos

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