PD-L1 Protein, Mouse, Recombinant (His Tag)

PD-L1 Protein, Mouse, Recombinant (His Tag): Product Information

Purity

> 98 % as determined by SDS-PAGE

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Activity

Measured by its binding ability in a functional ELISA. Immobilized recombinant mouse PD-L1 at 1 μg/ml (100 μl/well) can bind mouse PD1 with a linear range of 6.25-400 ng/ml.

Protein Construction

A DNA sequence encoding the mouse CD274 (NP_068693.1) extracellular domain (Met 1-Thr 238) was fused with a polyhistidine tag at the C-terminus.

Accession#

NP_068693.1

Expressed Host

HEK293 Cells

Species

Mouse

Predicted N Terminal

Phe 19

Molecule Mass

The secreted recombinant mouse CD274 comprises 231 amino acids and has a predicted molecular mass of 26.3 kDa. As a result of glycosylation, it migrates as an approximately 40-45 kDa band in SDS-PAGE under reducing conditions.

Formulation

Lyophilized from sterile PBS, pH 7.4

Please contact us for any concerns or special requirements.

Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

Please refer to the specific buffer information in the hard copy of CoA.