Phosphoglycerate Kinase Activity Assay Kit (Colorimetric)

Alternate Name : PGK1,Phosphoglycerate Kinase

Tag Line :

First kit in the market to measure PGK activity in biological samples

Storage Conditions: -20°C

Shelf life: 12 moths

Summary :

Phosphoglycerate Kinase Activity Assay Kit: Colorimetric Assay for Measuring PGK Activity in various Biological Samples such as Tissue/cell lysate. Convenient, Accurate & Reliable. Detection Limit: 50 mU. 100 Assays.

Detection Method: Colorimetric (OD = 340 nm)

Features & Benefits:

Sensitive (~ 50 mU),Fast & Convenient,Protocol takes ~60 minute to be completed,Simple procedure

Description:

Phosphoglycerate Kinase (PGK1) (EC 2.7.2.3) participates in two of the most important biochemical pathways in living organisms: Glycolysis and Gluconeogenesis. PGK is responsible for the catalysis of the reversible reaction of 1,3-Bisphosphoglycerate (1,3-BPG) and ADP to 3-Phosphoglycerate (3-PG) and ATP. In humans, PGK1 and PGK2, two PGK isozymes, have been characterized. While PGK1 is X-Chromosome-linked variant and expressed in all cells, PGK2 is autosome-linked variant and uniquely expressed in spermatogenic cells. Recent studies have shown deficiency of Phosphoglycerate Kinase could lead to chronic hemolytic anemia, mental disorders and myopathy in humans, whereas PGK overexpression promotes gastric cancer cell invasiveness. Therefore, accurate measurement of Phosphoglycerate Kinase Activity is useful for mechanistic and possible diagnostic studies. BioVision’s PGK Activity Assay kit provides a quick and easy way for monitoring PGK activity in various samples. In the first step of this enzymatic assay, PGK converts 3-Phosphoglycerate and ATP to 1,3-Bisphosphoglycerate and ADP. The nascent intermediate is detected via a series of enzymatic reactions that lead the oxidation of NADH to NAD+, which can be easily detected.