PMSF

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PMSF (Phenylmethyl sulfonyl fluoride) is an irreversible serine/cysteine protease inhibitor. PMSF rapidly inactivates purified chymotrypsin from human pancreas, although human trypsin is less susceptible to inhibition by PMSF. C14-labeled PMSF penetrates human red cells rapidly and inhibits acetylcholinesterase in situ. PMSF is known as phospholipase C inhibitor and also as acetylcholine esterase inhibitor. PMSF inhibits arachidonic acid release during first 4 minutes of ischemia. PMSF inhibits acetylcholine esterase activity in the early stage of complete cerebral ischemia, and induces an inhibition of increase of extracellular glutamate level. PMSF inhibits the addition of ethanolamine phosphate to glycosylphosphatidylinositol (GPI) intermediates in Trypanosoma brucei. PMSF also inhibits the acylation of the inositol residue of GPI intermediates in bloodstream form T. brucei. PMSF is capable of altering the actions of anandamide in vitro by blocking its metabolism. Administration of PMSF in vivo produces cannabinoid effects by altering endogenous levels of anandamide and potentiates the effects of exogenously administered anandamide. [4] Administration of PMSF can induce both promotion and protection in organophosphorus ester-induced delayed neuropathy (OPIDN) in hens. Pretreatment with PMSF results in a leftward shift of the anandamide dose effect curves for antinociception and hypothermia in male mice. Acetylcholinesterase (AChE), a serine hydrolase, is potentially susceptible to inactivation by PMSF. Pretreatment with PMSF protects against the degradation of neurofilaments in tri-ortho-cresyl phosphate (TOCP) induced delayed neuropathy in hens. PMSF treatment enhances the behavioral effects of endogenous and exogenous cannabinoid agonists in mice or rats.

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