Proinsulin ELISA

Brand

ALPCO

Description

pProinsulin ELISA/p
pimg alt=For In-Vitro Diagnostic Use src=http://www.alpco.com/img/ivd.gif align=absMiddle / For In-Vitro Diagnostic Use/p
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div class=prod_specbFormat:/b/div
div class=prod_spec_infoELISA / EIA/div
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div class=prod_specSample Type:/div
div class=prod_spec_infoserum, plasma/div
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div class=prod_specbSample Size:/b/div
div class=prod_spec_info50 micro;l/div
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div class=prod_specbSize:/b/div
div class=prod_spec_info96 wells/div
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div class=prod_specbRange:/b/div
div class=prod_spec_info2.5 – 180 pM/div
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div class=prod_specbSensitivity:/b/div
div class=prod_spec_info1.25 pM/div
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div class=prod_specbIncubation Time:/b/div
div class=prod_spec_info2 Hour/div
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pbSummary:/b/p
The ALPCO human Beta Cell line of Insulin, C-Peptide, and Proinsulin ELISAs are designed to meet your sample needs. The ALPCO Proinsulin sandwich ELISA is designed for the quantitative determination of proinsulin in Serum or Plasma. The ALPCO Insulin ELISA is a sandwich type immunoassay. The 96 well microplate is coated with a monoclonal antibody specific for insulin. The Insulin Standards, Diabetes Antigen Controls and samples are added to the microplate wells with a horseradish peroxidase enzyme labeled monoclonal antibody. The microplate is then incubated on an orbital microplate shaker at 700-900 rpm. After the first incubation is complete, the wells are washed with Wash Buffer and blotted dry. Substrate is added and the microplate is again incubated on an orbital microplate shaker (700-900 rpm). Once the second incubation period is complete, Stop Solution is added and the optical density (OD) is measured by spectrophotometer at 450nm with a reference wavelength of 620nm. The intensity of the color generated is directly proportional to the amount of insulin in the sample. The kit includes all components (96 well Ab coated plate, conjugate Ab, substrate, standards, quality controls and dilution and wash buffer) necessary to complete a the ELISA./div
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Application

Reactivity

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