Protein A Agarose Beads

Product Usage Information

Vortex tube briefly to resuspend the beads. Add 20 μl of bead slurry to each immunoprecipitation (IP) reaction. For bead washing and subsequent elution of immunocomplexes, the beads can be separated from solution by a brief 1 minute centrifugation in a microcentrifuge at 6,000 rpm. Resuspend the beads in solution by gently vortexing or rocking the tube. Please follow CST’s recommended IP protocol to perform IP followed by western blot.

Storage

Supplied as a 50% slurry in 18.5% ethanol solution. This product is stable for 12 months when stored at 4°C.

Product Description

Protein A Agarose Beads are an affinity matrix for the small-scale isolation of immunocomplexes from immunoprecipitations (IP assays). Protein A is covalently coupled to agarose beads. Protein A exhibits high affinity for subclasses of IgG from many species (including human, rabbit, mouse, rat, and sheep) and can be used for immunoprecipitation assays with these antibodies.

Product Specifications:

Bead Diameter: 45-165 micron per bead

Binding Capacity: 20 +/- 2 mg human IgG/ml