Rabbit antibody to GFP: affinity purified

ZZZZ_Status (Active, Disc) : Active

AA_Supplier Name : Biosensis

Alternative_Name (Other Names) :

Aequorea victoria green fluorescent protein

Antibody_Isotype
(Antibody only) : IgG

Application_Details :

Immunocytochemistry (1:2,000 – 1:5,000) and Western Blot (1:1,000 – 1:5,000). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.

Background_Info : The green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9 kDa) that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. Similar proteins that also fluoresce green are found in many marine organisms, but the label GFP traditionally refers to this particular protein, which was first isolated from the jellyfish Aequorea victoria and is sometimes called—when such precision is required—avGFP.The GFP from A. victoria has a major excitation peak at a wavelength of 395 nm and a minor one at 475 nm. Its emission peak is at 509 nm, which is in the lower green portion of the visible spectrum. The fluorescence quantum yield (QY) of GFP is 0.79. The GFP from the sea pansy (Renilla reniformis) has a single major excitation peak at 498 nm. GFP makes for an excellent tool in many forms of biology due to its ability to form internal chromophore without requiring any accessory cofactors, gene products, or enzymes / substrates other than molecular oxygen. https://en.wikipedia.org/wiki/Green_fluorescent_protein#:~:text=Similar%20proteins%20that%20also%20fluoresce,such%20precision%20is%20required%E2%80%94avGFP.

Clone_Name
(AB only, monoclonals) :

Datasheet_Link : https://www.biosensis.com/pdf_datasheet.php?products_id=1732

Description (Long Description) : 

The green fluorescent protein (GFP) is a 27 kDa protein isolated originally from the jellyfish Aequoria victoria. It has an endogenous fluorochrome activity with excitation maximum at 395nm and emission maximum at 509 nm, which is similar to that of fluorescein. GFP can be expressed in fluorescent form in essentially any prokaryotic or eukaryotic cell. This GFP rabbit antibody was made against a recombinant GFP construct originating from an Aequoria species which was engineered to improve spectral properties and prevent oligomerization (1). This form of GFP, referred to as AcGFP, is 94% identical to the eGFP developed by Tsien and co-workers. The antibody can be used to verify the expression, size and stability of both AcGFP and eGFP fusion proteins in western blotting experiments and to amplify GFP signals in tissues of transgenic animals.

Expiry_Date : 

12 months after purchase if unopened

Format (Form) : 

Lyophilized from a solution containing PBS, pH 7.2-7.6, 0.1% trehalose, 5 mM sodium azide as preservative.

Immunogen (Antigen) :Recombinant AcGFP purified from E. coli

Kit_Components :

MSDS (File name) :https://www.biosensis.com/pdf_MSDS.php?products_id=1732