Rabbit polyclonal antibody to human Protein kinase C alpha (153-182): Affinity purified

ZZZZ_Status (Active, Disc) : Active

AA_Supplier Name : Biosensis

Alternative_Name (Other Names) :

PKC-alpha; PKC-A; EC 2.7.11.13; PRKCA; PKCA; PRKACA;

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Application_Details :

Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.1-0.5µg/mL is recommended for WB. Human Protein kinase C alpha has a predicted length of 672 residues and MW of 77 kDa. A concentration of 0.5-1µg/mL is recommended to detect the protein in paraffin embedded tissues. Antigen retrieval by boiling paraffin sections in 10 mM citrate buffer, pH 6.0 for 20 minutes is required. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.

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Datasheet_Link : http://www.biosensis.com/pdf_datasheet.php?products_id=1569

Description (Long Description) : 

Protein kinase C alpha (PKC-alpha) belongs to the Protein kinase C family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol.  PKC-alpha is one of at least 12 different types of Protein kinase C identified in humans. PKC-alpha is ubiquitously expressed and has roles in many different cellular processes including cell adhesion, cell transformation, proliferation, differentiation and cell cycle checkpoint.

Expiry_Date : 

12 months after purchase (lyophilized)

Format (Form) : 

Lyophilized with 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3

Immunogen (Antigen) :A synthetic peptide corresponding to a sequence of human PRKCA (153-182aa MDHTEKRGRIYLKAEVADEKLHVTVRDAKN), that differs from the related rat and mouse sequences by one amino acid. There is 100% alignment with various monkey species including Rhesus, Baboon, gorillas by BLAST as well as other mammals including horse and marmoset.

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