Rat CD86 / B7-2 ELISA Pair Set
SEK80025
Brand
Sino Biological
Description
分子 : CD86
保存條件:
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20℃ to -80℃ and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
線性範圍 : 4.69-300
包被抗體 : 0.4 mg/mL of mouse anti-rat CD86 monoclonal antibody. Dilute to a working concentration of 2 μg/mL in CBS before coating. (Catalog: # 80025-MM12)
檢測抗體: 0.25 mg/mL mouse anti-rat CD86 monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 0. 5 μg/mL in detection antibody dilution buffer before use. (Catalog: # 80025-MM10)
標準品 : Each vial contains 19 ng of recombinant rat CD86. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 0.3 ng/mL is recommended.
生物素交聯HRP:
靈敏度 : The minimum detectable dose of Rat CD86 / B7-2 was determined to be approximately 4.7 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
特異性 :
檢測原理 : The Rat CD86 / B7-2 ELISA Pair Set is for the quantitative determination of Rat CD86 / B7-2.
This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs.
The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for CD86 / B7-2 coated on a 96-well plate. Standards and samples are added to the wells, and any CD86 / B7-2 present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated mouse anti-CD86 / B7-2 monoclonal antibody is then added, producing an antibody-antigen-antibody “sandwich”. The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of CD86 / B7-2 present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.
檢測限: 4.7 pg/ml
血清樣本檢測範圍 :
Application
Reactivity