Rat ICAM-1 / CD54 ELISA Pair Set

分子 : ICAM1

保存條件:

Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20℃ to -80℃ and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.

線性範圍 : 31.25-2000

包被抗體 : 0.5 mg/mL of mouse anti-rat ICAM1 monoclonal antibody. Dilute to a working concentration of 2 μg/mL in CBS before coating. (Catalog: # 80022-R037)

檢測抗體: 0.5 mg/mL mouse anti-rat ICAM1 monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use. (Catalog: # 80022-R112)

標準品 : Each vial contains 60 ng of recombinant rat ICAM1. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 2 ng/mL is recommended.

生物素交聯HRP:

靈敏度 : The minimum detectable dose of Rat ICAM-1 / CD54 was determined to be approximately 31.25 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.

特異性 :

檢測原理 : The Rat ICAM-1 / CD54 ELISA Pair Set is for the quantitative determination of Rat ICAM-1 / CD54.
This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs.
The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for ICAM-1 / CD54 coated on a 96-well plate. Standards and samples are added to the wells, and any ICAM-1 / CD54 present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated mouse anti-ICAM-1 / CD54 monoclonal antibody is then added, producing an antibody-antigen-antibody “sandwich”. The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of ICAM-1 / CD54 present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.

檢測限: 31.25 pg/ml

血清樣本檢測範圍 :