Recombinant Protein G Protein, His Tag

Source : 

Recombinant Protein G, His Tag, also known as Recombinant Staphylococcus Aureus protein G148, was expressed in E.Coli at ACRObiosystems.

Molecule : Protein G

Synonyms : RPG,Protein G

Format : Powder

Category : MABSol® Affinity Media Ligands

Accession : N/A

Storage : -20℃

Shipping condition : Powder,RT

Molecular Weight : N/A

Characteristics :

Recombinant Protein G, His Tag is fused with the polyhistidine tag at N-terminus and a single cysteine at C-terminus. The reducing (R) protein migrates as a 29 kDa polypeptide. Protein G Protein can bind to human IgG1 Fc.

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

Buffer : 100 mM NaH2PO4, pH8.5

Description :

Protein G is an immunoglobulin-binding protein expressed in group C and G Streptococcal bacteria much like Protein A but with differing specificities. It is a 65-kDa (G148 protein G) and a 58 kDa (C40 protein G) cell surface protein that has found application in purifying antibodies through its binding to the Fc region. The native molecule also binds albumin, however, because serum albumin is a major contaminant of antibody sources, the albumin binding site has been removed from this recombinant form of Protein G.

References :

(1) Sjobring U, Bjorck L, Kastern W, et al., 1991, J Biol Chem 266 (1): 399–405.