保存條件 : Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
蛋白構建: A DNA sequence encoding the rhesus IL12A (NP_001038199.1) (Met1-Ser219) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the rhesus IL12B (NP_001038190.1) (Met1-Ser328) was expressed with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the rhesus IL12A & the rhesus IL12B heterodimer was purified.
纯度: > 95 % as determined by SDS-PAGE
内毒素 : < 1.0 EU per μg of the protein as determined by the LAL method