Rhesus macaque TFPI Protein, His Tag

Source : 

Rhesus macaque TFPI, His Tag (TFI-C52H3) is expressed from human 293 cells (HEK293). It contains AA Asp 29 – Lys 282 (Accession # Q28864).

Molecule : TFPI

Synonyms : TFPI,LACI,TFPI1,EPI,TFI

Format : Powder

Category : Other Recombinant Proteins

Accession : N/A

Storage : -20℃

Shipping condition : Powder,RT

Molecular Weight : 31.1 kDa

Characteristics :

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 31.1 kDa. The protein migrates as 41-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

Buffer : PBS, pH7.4

Description :

Tissue factor pathway inhibitor (TFPI) is also known as Extrinsic pathway inhibitor (EPI), Lipoprotein – associated coagulation inhibitor (LACI), is a plasma proteinase inhibitor synthesized by vascular endothelial cells and part of it is associated with glycosaminoglycans of these cells. TFPI is a single-chain polypeptide which can reversibly inhibit Factor Xa (Xa) and Thrombin (Factor IIa). TFPI is a secreted protein with a N­terminal acidic region, three Kunitz (K) domains separated with by two linker regions, and a C­terminal basic region. The first K domain inhibits coagulation factor VIIa complexed to tissue factor (TF); The second K domain inhibits factor Xa; The third K domain binds to heparin; The C­terminal basic region may have several functions. For example, it plays an important role in binding of TFPI to cell surfaces.

References :

(1) Bajaj MS., et al., 2001, Thromb Haemost., 86:959.
(2) Mine S., et al., 2002, Biochemistry, 41:78.
(3) Witt I., et al., 2002, Hamostaseologie, 22:30–35.