Purity : > 95% as determined by SEC-HPLC Analysis.
Reconstitution : Stock solution (0.1 mg/mL): Dissolve the powder with 100 µL DMSO and 400 µL PBS or sterile water .
Please note that the final concentration of DMSO must be below 1 % (v/v) to avoid toxicity in the biological system.
Storage: Lyophilized powder: -20℃ for 12 months upon receipt.
After reconstitution, please store the stock solution at -80℃, and it should be aliquoted to avoid repeated freeze-thaw cycles. It is recommended to use up within 3 months.
Traits : Liquid
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Coronavirus spike Background Information :
The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2; DPP4, dipeptidyl peptidase-4; APN, aminopeptidase N; CEACAM, carcinoembryonic antigen-related cell adhesion molecule 1; Sia, sialic acid; O-ac Sia, O-acetylated sialic acid. The spike is essential for both host specificity and viral infectivity. The term ‘peplomer’ is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It’s been reported that SARS-CoV-2 (COVID-19 coronavirus, 2019-nCoV) can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate.
Antigen specific T-cell stimulationCellular immune responseImmune monitoringT-cell assaysT-cell expansion