KitsTotal Carbohydrate Colorimetric Assay Kit

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K645-100

K645-100

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Description

Alternate Name : Total Saccharide Assay Kit

 

Tag Line :

Robust, Sensitive Assay

 

Storage Conditions: 4°C

 

Shelf life: 12 months

 

Summary :

• Detection method- Absorbance (490 nm)
• Can detect most forms of carbohydrates, including simple & complex saccharides, glycans, glycoproteins & glycolipids

 

Detection Method:

 

Features & Benefits:

• Simple procedure; takes ~ 30 minutes
• Rapid, convenient and sensitive
• Kit contains all necessary reagents for measuring total carbohydrate concentration

 

Description:

Carbohydrates play important structural as well as chemical roles in all living systems. Detection of total carbohydrates, therefore, has wide applications. BioVision’s Total Carbohydrate Assay is a simple, sensitive and robust method of detecting virtually all carbohydrates. The assay is based on the phenol-sulfuric acid method. In Total Carbohydrate Assay, polysaccharides (mono, di, tri, etc.) and their derivatives, in the presence of sulfuric acid, are hydrolyzed to monomers and converted to furfural or hydroxyfurfural, which react with the Developer to form a chromogen that can be quantified by measuring the absorbance at 490 nm. The Total Carbohydrate Assay can detect most forms of carbohydrates, including simple and complex saccharides, glycans, glycoproteins and glycolipids.

 

Application

Reactivity

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