Ubiquitin Activating Enzyme (UBE1)

PDF

E-305

25 μg

Brand

Boston Biochem

Description

Ubiquitin Activating Enzyme (UBE1)

Catalog #: E-305

Ubiquitin Activating Enzyme (UBE1)
Product Source: 

human reconbinant

Also known as: UBA1

This enzyme is responsible for the first step in ubiquitin-protein isopeptide bond formation, by forming a high-energy thioester bond with ubiquitin. The ubiquitin is activated by first adenylating with ATP its C-terminal glycine residue (Gly76) and thereafter linking this residue to the side chains of a cysteine residue in UBE1, yielding an ubiquitin-UBE1 thiolester and free AMP and PPi. The activated ubiquitin is then transferred to a lysine of the targeted protein via the E2-E3 conjugation cascade. UBE1 is a critical component for the initiation of any in vitro conjugation reactions.

Product Information
Quantity: 

25 μg

Stock: 

X mg/ml (X μM) in 50 mM HEPES pH 8.0. Actual concentration varies with lot number

MW: 

110 kDa

Purity: 

> 95% by SDS-PAGE

 

Use & Storage
Use: 

Typical enzyme concentration to support conjugation in vitro is 50-200 nM depending on conditions.

Storage: 

Store at –80°C. Avoid multiple freeze/thaw cycles.

Application

Reactivity

Photos

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