UCH-L1

PDF

E-340

25 Î¼g

Brand

Boston Biochem

Description

UCH-L1

Catalog #: E-340

UCH-L1

Product Source:

human recombinant

Also known as: Ubiquitin C-terminal Hydrolase L1 (UCH-L1)

Ubiquitin C-terminal hydrolases are a family of cysteine hydrolases that catalyze the hydrolysis of amides, esters and thioesters from the C-terminus of ubiquitin. UCH-L1 is a member of the low molecular weight UCHs that processes ubiquitin precursors and ubiquitinated proteins to generate monomeric ubiquitin. The expression of UCH-L1 is highly specific to cells of the neuroendocrine system and their tumors. UCH-L1 variants (Ile⁹³Met) have been linked to Parkinson disease and may participate in processes critical for neuronal health.

Product Information

Quantity:

25 μg

Stock:

X mg/ml (X μM) in 50 mM HEPES pH 8.0, 100 mM NaCl, 1 mM EDTA, 2 mM DTT. Actual protein concentration will vary with specific Lot #.

MW:

25 kDa

Purity:

> 95% by SDS-PAGE

Use & Storage

Use:

Typical enzyme concentration to support hydrolysis of substrates in vitro ranges from 10-100 nM depending on conditions and substrate. Pre-incubation (15 min) with 10 mM DTT is recommended to achieve maximum activity.

Storage:

Store at -80°C. Avoid multiple freeze/thaw cycles.