Z-Leu-Arg-Gly-Gly-AMC (Z-LRGG-AMC)

PDF

S-100

5 mg

Brand

Boston Biochem

Description

Z-Leu-Arg-Gly-Gly-AMC (Z-LRGG-AMC)

Catalog #: S-100

Product Source:

human recombinant

Fluorgenic tetra-peptide substrate for ubiquitin C-terminal hydrolases (UCHs, eg. UCHL3) and Isopeptidase T. This low molecular weight peptide represents a minimal sequence of C-terminal residues of ubiquitin where hydrolysis occurs at the UbGly⁷⁶-X bond. The catalytic efficiency for hydrolysis of this substrate by deconjugating enzymes is several thousand-fold less than a more native substrate (Ub-AMC, Cat # U-550) since remote interactions with Ub-containing substrates stabilize catalytic transition states.

Product Information

Quantity:

5 mg

MW:

11 kDa

Structure:

Purity:

> 95% by HPLC

Solubility:

Soluble in DMSO. For best results, pellet dry compound prior to reconstitution. Solubilize at desired stock concentration.

Western Blot Data:

Use & Storage

Use:

Add from DMSO stock directly to in vitro or in vivo assay at desired concentration. Typical concentrations range from 10-100 μM. (λ_ex: 380nm; λ_em: 460 nm)

Storage:

Store lyophilized powder or stock solution at -20°C. Avoid multiple freeze/thaw cycles.