Zika virus (strain Zika SPH2015) Envelope Protein (ZIKV-E) ELISA Pair Set

分子 : ZIKV-E

保存條件:

Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.

線性範圍 : 125-8000

包被抗體 : 0.5 mg/mL of mouse anti-Zika virus (strain Zika SPH2015) Envelope Protein (ZIKV-E) monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 μg/mL in PBS before coating. (Catalog: # 40543-MM35)

檢測抗體: 0.2 mg/mL of rabbit anti-Zika virus (strain Zika SPH2015) Envelope Protein (ZIKV-E) monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.8 μg/mL in detection antibody dilution buffer before use. (Catalog: # 40543-R033)

標準品 : Each vial contains 510 ng of recombinant Zika virus (strain Zika SPH2015) Envelope Protein (ZIKV-E). Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 8000 pg/mL is recommended.

生物素交聯HRP:

靈敏度 : The minimum detectable dose of Zika virus (strain Zika SPH2015) Envelope Protein (ZIKV-E) was determined to be approximately 125 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.

特異性 :

檢測原理 : The Zika virus (strain Zika SPH2015) Envelope Protein (ZIKV-E) ELISA Pair Set is for the quantitative determination of Zika virus (strain Zika SPH2015) Envelope Protein (ZIKV-E).
The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for Zika virus (strain Zika SPH2015) Envelope Protein (ZIKV-E) coated on a 96-well plate. Standards and samples are added to the wells, and any Zika virus (strain Zika SPH2015) Envelope Protein (ZIKV-E) present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated rabbit anti-Zika virus (strain Zika SPH2015) Envelope Protein (ZIKV-E) monoclonal antibody is then added, producing an antibody-antigen-antibody “sandwich”. The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of Zika virus (strain Zika SPH2015) Envelope Protein (ZIKV-E) present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.

檢測限: 125 pg/ml

血清樣本檢測範圍 :