Sino biological | 新冠病毒中和抗體/S2-特異性抗體

Sino biological | 新冠病毒中和抗體/S2-特異性抗體

2020.05.25

 【新冠病毒中和抗體|S2-特異性抗體】

SARS-CoV-2
Neutralizing Antibodies | S2-Specific Antibodies

Antibody Whitepaper

To support COVID-19 research, Sino Biological has developed a large collection of antibodies against different antigens of SARS-CoV-2. These antibodies can be used to detect and/or neutralize the virus in a variety of assays including ELISA, western blot, immunofluorescent staining, immunohistochemistry, SPR (Biacore and Octet), pseudovirus infection, and potentially in vivo animal studies.

This week, Sino Biological announces the new additions to this product line:

The publication of SARS-CoV-2 antibody whitepaper.

This guidebook will help the users find the most appropriate antibodies for their assay.

The launch of two mouse mAbs with strong neutralizing activities.

These antibodies are SARS-CoV-2 specific, with no cross-reactivity with SARS-CoV, and other common coronaviruses.

The launch of a chimeric antibody specifically recognizing the S2 domain of SARS-CoV-2.

Full List of SARS-CoV-2 Antibodies

 

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Get Free Antibody Guidebook

Specific and Neutralizing Antibodies against SARS-CoV-2 Spike

Cat #

40591-MM43

40592-MM57

Description

Anti-Spike S1

Anti-Spike RBD

Type

Mouse MAb

Mouse MAb

Ig Type

Mouse IgG1

Mouse IgG2b

Application

Neutralization, ELISA

Neutralization, ELISA

Neutralization Activity Assay

Neutralizing antibodies can effectively inhibit the infection of SARS-CoV-2 Spike pseudovirus on ACE2 overexpression 293T cells.

S1 Neutralizing Antibody

 

RBD Neutralizing Antibody

Conc. (µg/mL)

Inhibition%

Conc. (µg/mL)

Inhibition%

100

96.39%

100

95.51%

10

94.57%

10

93.32%

1

42.24%

1

75.69%

0.1

-5.53%

0.1

10.11%

Competitive-ELISA Assay

Neutralizing ability was detected by SARS-CoV-2 Inhibitor Screening ELISA Kit (Catalog# KIT001) using competitive ELISA.

S1 Neutralizing Antibody

 

RBD Neutralizing Antibody

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Specificity Assay

These two anti-spike antibodies have been tested against all seven coronaviruses, and are shown to be specific to SARS-CoV-2 spike. The reactivity of the antibodies against the known coronaviruses is assessed in ELISA. The reactivity (OD450) is illustrated in the figures below.

S1 Neutralizing Antibody

 

RBD Neutralizing Antibody

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Antibody Against SARS-CoV-2 Spike S2

Cat #

40590-D001

Description

SARS-CoV-2 Spike S2 Antibody

Type

Chimeric MAb

Ig Type

Mouse V domain & Human IgG1

Cross-reactivity

No cross-reactivity in ELISA with
SARS-CoV-2 (2019-nCoV) Spike S1 (Cat# 40591-V08H)
SARS-CoV-2 (2019-nCoV) Spike RBD (Cat# 40592-V05H)

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