亞旭生物科技

類器官(organoid)是由多個細胞組成3D的立體結構組織，透過體外的3D組織培養技術可以模擬器官組織在體內的生長條件。與簡單的傳統型2D細胞培養相比，3D組織培養多了細胞與細胞之間以及細胞與細胞外基質之間的交互作用，因此類器官3D的培養環境會更符合在人體內的生長環境。綜合上述優勢現今越來越多研究開始改變培養模式，類器官培養近期也成為生物研究的一個重要工具。

類器官的來源可以自胚胎幹細胞 (ESC)、誘導性全能幹細胞(iPSC) 或初代組織。不同的細胞類型和要後續要分化類型都需要不同的成分的培養基，而培養基中會需要生長因子，如EGF, Noggin (NOG), R-spondin (RSPO1), HGF, BMP和 FGF, 以上皆是類器官培養基的重要成分。

Type of Tissue	Source	Stem Cell Culture Condition (niche factors)	Differentiation Culture Condition
Stomach	Adult mouse	EGF, Noggin, R-spondin, Wnt-3A, FGF10	EGF, R-spondin EGF, R-spondin
	Adult human	EGF, Noggin, R-spondin, Wnt-3A, FGF10
	hPSC	EGF	EGF

Small Intestine	Adult mouse	EGF, Noggin, R-spondin	EGF, Noggin, R-spondin
	Adult human	EGF, Noggin, R-spondin, Wnt-3A TGF-beta inhibitor, p38 inhibitor	EGF, Noggin, R-spondin, TGF-beta inhibitor
	hPSC	EGF	EGF

Colon	Adult mouse	EGF, Noggin, R-spondin, Wnt-3A	EGF, Noggin, R-spondin
Colon	Adult human	EGF, Noggin, R-Spondin, Wnt-3A, TGF-beta inhibitor, p38 inhibitor	EGF, Noggin, R-spondin, TGF-beta inhibitor

Pancreas	Adult mouse	EGF, Noggin, R-spondin, Wnt-3A, FGF10, Nicotinamide	EGF, Noggin, R-spondin, Wnt-3A
Pancreas	Adult human	EGF, Noggin, R-spondin, Wnt-3A, FGF10, TGF-beta inhibition, Nicotinamide	Not reported

Liver	Adult mouse	EGF, Noggin, R-spondin, Wnt-3A, FGF10, HGF, Nicotinamide	EGF, Noggin, FGF10, TGF-beta inhibition, Notch inhibition
Liver	Adult human	EGF, Noggin, R-spondin, Wnt-3A, FGF10, HGF, Nicotinamide, TGF-b inhibitor, Forskolin	EGF, Noggin, FGF10, TGF-beta inhibition, Notch inhibition, BMP7

Table 1: Culture Conditions for Organoids from Stem Cells (Source: Cell)

Sino Biological has developed a panel of recombinant growth factors with high bioactivity, batch-to-batch consistency, and purity to enable optimal and consistent organoid growth.

用於類器官培養的重組生長因子蛋白

Sino Biological已開發生產多物種(人類、小鼠、獼猴、恒河猴、大鼠和犬類)具有生物活性的重組生長因子。

• EGF Protein

Human EGF Protein: 10605-HNAE

High-purity		Low-endotoxin

Tag-free		HPLC-verified

> 95 % as determined by SEC-HPLC

A cell proliferation assay using Balb/C 3T3 mouse embryonic fibroblasts

Batch-to-batch Consistency

• 其他生長因子

Recombinat HGF Proteins
Human HGF Protein \| 10463-HNAS	Cynomolgus HGF Protein \| 90286-CNAH

Ability to neutralize TGF-beta mediated inhibition on Mv-1-Lu cell proliferation.	Ability to inhibit TGFβ1 activity on Mv-1-lu mink lung epithelial cells.

Human FGF10 Protein \| 10573-HNAE	Human Noggin/NOG Protein \| 10267-HNAH

Cell proliferation assay using BaF3 mouse proB cells transfected with human FGFR2b.	Ability to inhibit BMP4-induced alkaline phosphatase production by MC3T3E1 mouse preosteoblast cells.

Human RSPO1 Protein \| 11083-HNAS	Human BMP-2 Protein \| 10426-HNAE

Ability to induce activation of ßcatenin response in a Topflash Luciferase assay using HEK293T human embryonic kidney cells.	Ability to induce alkaline phosphatase production by MC3T3-E1 mouse osteoblastic cells.

更多用於類器官培養的重組生長因子

EGF	FGF2	FGF7	FGF9	FGF10

HGF	NOG	RSPO1	RSPO3	Activin A

BMP2	BMP4

Sino Biological | 用於類器官(organoid)培養的重組生長因子蛋白

Sino Biological | 用於類器官(organoid)培養的重組生長因子蛋白

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