 |
|
 |
|
|
|
| 生產膜蛋白非常的困難且具有挑戰性，主要是受到低表達、製備困難和穩定性差的因素所影響。 Sino Biological利用HEK293表達系統的病毒樣顆粒virus-like particle (VLP)技術平台，提供客製化膜蛋白生產服務。 該平台在 VLP 表面顯示目標膜蛋白，允許將其作為正常蛋白質進行檢測。 |
|
|
The process of VLP displaying target protein
|
|
|
|
|
|
服務特色
|
|
|
|
|
|
|
|
快速
|
|
良好生物活性
|
|
多種應用 |
| 最快3-4週完成 |
HEK293細胞表達，
相似於天然結構 |
Immunization, ELISA, SPR/BLI, PK/PD, etc. |
|
|
|
|
服務流程
|
|
Step and Content
|
Timeline
|
Deliverables
|
|
 Gene synthesis and codon optimization (optional)
• Simply provide us with the protein/gene sequence or plasmid
|
2 weeks
|
• 50-200 μg purified proteins
(if feasible)
• CoA:
> BCA and WB
> SEC-HPLC (optional)
> ELISA (optional)
|
|
Vector construction
• Cloning into optimized and efficient expression vector
• Plasmid sequencing and large-scale plasmid preparation
|
1-2 weeks
|
|
Pilot-scale protein expression and purification
• Transient transfection of HEK293 cells
• VLP protein purification
• QC analysis
|
2-3 weeks
|
|
Large-scale expression and purification (optional)
• Protein expression and purification
• QC analysis
|
3-4 weeks
|
• Purified proteins
• CoA
|
|
|
|
| 案例分享：Claudin 6-VLP蛋白 |
| Sino Biological利用 VLP 的膜蛋白表達平台開發多種全長跨膜蛋白，此外活性也已經過驗證 |
|
Full-length Human Claudin 6-VLP Protein (Cat#: 16658-HNAH)
|
|
High Activity
|
|
High batch-to-batch Consistency
|
|
High Freeze-thaw Stability
|
|
|
|
|
|
|
更多全長跨膜VLP蛋白
|
|
|
|
|
|
|
