IL-6 is a pleiotropic cytokine. Elevated IL-6 is observed in patients with microbial infections and other inflammatory conditions. The serum level of IL-6 is often correlated with the severity of the inflammation.
The pro-inflammatory function of IL-6 is regulated by a trans-signaling process involving soluble IL-6R (sIL-6R) and gp130 receptor. The production of sIL6R is activated by IL-1beta and TNF-alpha. These two pro-inflammatory cytokines induce the proteolytic cleavage of IL-6R by activating ADAM10 and ADAM17.
Unlike most other soluble receptors (e.g. IL-1RA and IL-18BP), sIL-6R is NOT an inhibitor of IL-6. Instead, it activates IL-6 signaling by forming a complex with IL-6 and gp130.
Human IL-6R (cat#: 10398-H08H) enhanced the IL-6 activity on M1 mouse myeloid leukemia cells. The ED50 is typically 20-80 ng/mL.
In addition, IL-6 can transduce its signal through membrane-bound IL-6R. This signal transduction also requires gp130 to be the co-receptor.
Bioactive Recombinant IL-6 from Multiple Species
Cell Proliferation Assay Using TF-1 Human Erythroleukemic Cells. (cat#: 10395-HNAE)