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IL-6 is a pleiotropic cytokine. Elevated IL-6 is observed in patients with microbial infections and other inflammatory conditions. The serum level of IL-6 is often correlated with the severity of the inflammation.
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The pro-inflammatory function of IL-6 is regulated by a trans-signaling process involving soluble IL-6R (sIL-6R) and gp130 receptor. The production of sIL6R is activated by IL-1beta and TNF-alpha. These two pro-inflammatory cytokines induce the proteolytic cleavage of IL-6R by activating ADAM10 and ADAM17.
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Unlike most other soluble receptors (e.g. IL-1RA and IL-18BP), sIL-6R is NOT an inhibitor of IL-6. Instead, it activates IL-6 signaling by forming a complex with IL-6 and gp130.
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Human IL-6R (cat#: 10398-H08H) enhanced the IL-6 activity on M1 mouse myeloid leukemia cells. The ED50 is typically 20-80 ng/mL.
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In addition, IL-6 can transduce its signal through membrane-bound IL-6R. This signal transduction also requires gp130 to be the co-receptor.
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Bioactive Recombinant IL-6 from Multiple Species
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Recombinant Membrane-bound IL-6 Receptor: IL-6R
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IL-6 demonstrates limited cross-species bioactivity. Human IL-6 can bind to both human and mouse IL-6R, but mouse IL-6 can only bind to mouse IL-6R.
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Recombinant gp130
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Gp130 is a common receptor and signal transducer shared by most IL-6 family cytokines.
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ELISA Detection Pairs: IL-6 and IL-6R
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Sino Biological has developed high sensitivity ELISA antibody pairs for detection of IL-6 and IL-6R.
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Neutralization Antibodies: IL-6 and IL-6R
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IL-6 Neutralizing Antibody
(Cat#: 10395-R508)
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IL-6R Neutralizing Antibody
(Cat#: 10398-MM08)
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Neutralize IL-6 induced proliferation in the T1165 mouse plasmacytoma cells. The IC50 is typically 80-320ng/mL
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IL-6-dependent Inhibition of cell proliferation is neutralized by Human IL-6R neutralizing Monoclonal Antibody. The IC50 is typically 0.15-0.6 μg/mL.
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More IL-6 Family Cytokines
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