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SignalChem Biotech, now part of Sino Biological, has developed an extensive portfolio of
over
1500 recombinant active enzymes,
including clinically significant mutants. These enzymes are extensively used in biochemical assays, drug
discovery, and molecular biology research. Leveraging our deep expertise in enzyme and assay development,
we offer
tailored
contract research services including enzyme development, compound profiling, and assay
development,
to ensue precise solutions for your diverse research needs. |
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| Enzyme Development Services |
SignalChem Biotech specializes in crafting recombinant active enzymes, including kinases, phosphatases,
ubiquitin and epigenetic enzymes. Backed by over 20 years of expertise in enzyme production, we offer
customized services to develop
any recombinant enzyme promptly to meet various research demands. |
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Service Highlights
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Cloning
Primers are designed for gene amplification from human cDNA libraries
and cloned into vectors for optimal expression.
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Expression
An expression system is selected based on the client requirements,
considering necessary PTMs and specific enzymatic activity needs.
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Purification & Modification
Standard protocols are followed for purification, with options for
phosphorylation, tag removal, or buffer exchange provided as directed by the client.
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Characterization
The final product is tested to ensure meeting client-specified
standards for concentration, purity, and activity.
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| Compound Profiling Services |
SignalChem Biotech boasts one of the most comprehensive selections of active enzymes, including the
world’s largest active kinase catalog. Leveraging this extensive collection, we offer efficient
compound selectivity profiling services to determine the inhibitory profiles and
mechanisms of
action of drug candidates. |
| Service Highlights |
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1500+ Enzyme Targets
Kinases, phosphatases, epigenetic enzymes, and beyond |
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1100+ Active Kinases
• 650+ wild-type kinases
• 450+ clinically relevant mutant kinases |
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Fast Turnaround Time
Accurate results are provided within TWO WEEKS |
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Custom Assay Development & Flexible Setups
Including biomedical and cell-based assays |
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Extensive Kinase Expertise
Over 20 years of experience in kinase and assay development |
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Service Details and FAQs >>
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| Case Studies |
| 1 |
Ki Determination of Pan-HER Tyrosine Kinase Inhibitor Neratinib
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Kinase: wild-type HER2 (GST tag, cat#: E27-11G)
Substrate: polyEY (cat#: P61-58) |
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IC50 Determination of 4 Commercial TRK Inhibitors: Larotrectinib,
Selitrectinib, Repotrectinib, and Zurletrectinib
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Kinase: wild-type TRKC (His tag, cat#: N18-11H)
Substrate:
polyEY (cat#: P61-58) |
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