The transEDIT-dual  CRISPR-Cas9 Whole Genome Arrayed Library was developed using the CRoatan algorithm by employing a random-forest-based gRNA prediction tool to create novel and superior gRNA designs. These gRNAs were then paired in a lentiviral vector to co-express and thus further augment their potency. A molecular barcode was incorporated in the vector to allow for downstream high-throughput analysis.

Vector:

Advantages:

• CRoatan algorithm gRNA designs result in superior knockout efficiency (read publication)

• Two independent gRNAs in the same lentiviral vector further enhance for potent knockout

• Unique barcode sequence identifier enables downstream analysis

• All clones are sequence-verified

The transEDIT-dual CRISPR-Cas9 Whole Genome Arrayed Library targets over 19,000 genes in the human genome and is provided in 96-well plates as bacterial glycerol stocks.