Dual gRNA expression for superior knockout efficacy
Sequence-verified arrayed libraries are ideal resources for core labs
The transEDIT-dual CRISPR-Cas9 Whole Genome Arrayed Library was developed using the CRoatan algorithm by employing a random-forest-based gRNA prediction tool to create novel and superior gRNA designs. These gRNAs were then paired in a lentiviral vector to co-express and thus further augment their potency. A molecular barcode was incorporated in the vector to allow for downstream high-throughput analysis.
CRoatan algorithm gRNA designs result in superior knockout efficiency (read publication)
Two independent gRNAs in the same lentiviral vector further enhance for potent knockout